NAT10 antibody (Internal Region)

Catalog No. ABIN6263520

The Rabbit Polyclonal anti-NAT10 antibody has been validated for WB and IHC. It is suitable to detect NAT10 in samples from Human, Mouse and Rat.

Quick Overview for NAT10 antibody (Internal Region) (ABIN6263520)

Target: NAT10 (N-Acetyltransferase 10 (GCN5-Related) (NAT10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAT10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NAT10 Antibody

Binding Specificity: Internal Region

Specificity: NAT10 Antibody detects endogenous levels of total NAT10.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NAT10, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NAT10

Target: NAT10 (N-Acetyltransferase 10 (GCN5-Related) (NAT10))

Alternative Name: NAT10

Background:

Description: RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs (PubMed:25411247, PubMed:25653167). Catalyzes the formation of N4-acetylcytidine (ac4C) at position 1842 in 18S rRNA (PubMed:25411247). May also catalyze the formation of ac4C at position 1337 in 18S rRNA (By similarity). Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis (PubMed:25411247, PubMed:25653167). Catalyzes the formation of ac4C in serine and leucine tRNAs (By similarity). Requires the tRNA-binding adapter protein THUMBD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation (PubMed:25653167). Can acetylate both histones and microtubules (PubMed:14592445, PubMed:17631499, PubMed:19303003). Histone acetylation may regulate transcription and mitotic chromosome de-condensation (PubMed:17631499). Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization (PubMed:14592445, PubMed:18082603). Acetylates alpha-tubulin, which may affect microtubule stability and cell division (PubMed:19303003).

Gene: NAT10

Molecular Weight: 116 kDa

Gene ID: 55226

UniProt: Q9H0A0