NEK4 antibody (Internal Region)

Catalog No. ABIN6263592

The Rabbit Polyclonal anti-NEK4 antibody has been validated for ELISA, WB and IHC. It is suitable to detect NEK4 in samples from Human, Mouse and Rat.

Quick Overview for NEK4 antibody (Internal Region) (ABIN6263592)

Target: NEK4 (NIMA related kinase 4 (NEK4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NEK4 Antibody

Binding Specificity: Internal Region

Specificity: NEK4 Antibody detects endogenous levels of total NEK4.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NEK4, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NEK4

Target: NEK4 (NIMA related kinase 4 (NEK4))

Alternative Name: NEK4

Background:

Description: Protein kinase that seems to act exclusively upon threonine residues (By similarity). Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage.

Gene: NEK4

Molecular Weight: 95 kDa

Gene ID: 6787

UniProt: P51957