NUDT1 antibody (Internal Region)

Catalog No. ABIN6263763

Product Details anti-NUDT1 Antibody

Target: NUDT1 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 1 (NUDT1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUDT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: NUDT1 Antibody detects endogenous levels of total NUDT1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NUDT1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NUDT1

Target: NUDT1 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 1 (NUDT1))

Alternative Name: NUDT1 (NUDT1 Products)

Synonyms: mutT antibody, nudT1 antibody, MTH1 antibody, Mth1 antibody, 7,8-dihydro-8-oxoguanine triphosphatase antibody, nudix hydrolase 1 antibody, nudix (nucleoside diphosphate linked moiety X)-type motif 1 antibody, mth1 antibody, Tsp_07498 antibody, nudt1 antibody, 8odp antibody, NUDT1 antibody, Nudt1 antibody

Background:

Description: Antimutagenic (PubMed:8226881, PubMed:7713500, PubMed:10608900). Plays a redundant role in sanitizing oxidized nucleotide pools, such as 8-oxo-dGTP pools (PubMed:28679043). Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress (PubMed:12857738, PubMed:24695224, PubMed:24695225). Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions (PubMed:8226881, PubMed:10373420, PubMed:10608900, PubMed:11756418, PubMed:12857738, PubMed:16607562, PubMed:24695224, PubMed:24695225, PubMed:26999531, PubMed:28035004). Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP (PubMed:10373420, PubMed:11139615). Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA (By similarity).

Gene: NUDT1

Molecular Weight: 22kDa

Gene ID: 4521

UniProt: P36639