P4HB antibody (C-Term)

Catalog No. ABIN6263967

The Rabbit Polyclonal anti-P4HB antibody has been validated for WB, IHC and ELISA. It is suitable to detect P4HB in samples from Human, Mouse and Rat.

Quick Overview for P4HB antibody (C-Term) (ABIN6263967)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-P4HB Antibody

Binding Specificity: C-Term

Specificity: P4HB Antibody detects endogenous levels of total P4HB.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human P4HB, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: P4HB

Background:

Description: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9, the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).

Gene: P4HB

Molecular Weight: 57kDa

Gene ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism