PDLIM1 antibody (Internal Region)

Catalog No. ABIN6264117

The Rabbit Polyclonal anti-PDLIM1 antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect PDLIM1 in samples from Human, Mouse and Rat.

Quick Overview for PDLIM1 antibody (Internal Region) (ABIN6264117)

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDLIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PDLIM1 Antibody

Binding Specificity: Internal Region

Specificity: PDLIM1 Antibody detects endogenous levels of total PDLIM1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PDLIM1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, IF/ICC 1:200-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PDLIM1

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Alternative Name: PDLIM1

Background:

Description: Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed:10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).

Gene: PDLIM1

Molecular Weight: 36 kDa

Gene ID: 9124

UniProt: O00151