SLC26A5 antibody (Internal Region)

Catalog No. ABIN6264415

This anti-SLC26A5 antibody is a Rabbit Polyclonal antibody detecting SLC26A5 in WB, ELISA, IHC, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for SLC26A5 antibody (Internal Region) (ABIN6264415)

Target: SLC26A5 (Solute Carrier Family 26, Member 5 (Prestin) (SLC26A5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC26A5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SLC26A5 Antibody

Binding Specificity: Internal Region

Specificity: Prestin Antibody detects endogenous levels of total Prestin.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Prestin, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SLC26A5

Target: SLC26A5 (Solute Carrier Family 26, Member 5 (Prestin) (SLC26A5))

Alternative Name: SLC26A5

Background:

Description: Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site (By similarity).

Gene: SLC26A5

Molecular Weight: 81 kDa

Gene ID: 375611

UniProt: P58743

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport