Ribonuclease H1 antibody (Internal Region)

Catalog No. ABIN6264778

This anti-Ribonuclease H1 antibody is a Rabbit Polyclonal antibody detecting Ribonuclease H1 in WB, IHC, ICC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for Ribonuclease H1 antibody (Internal Region) (ABIN6264778)

Target: Ribonuclease H1 (RNASEH1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ribonuclease H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Ribonuclease H1 Antibody

Binding Specificity: Internal Region

Specificity: RNase H1 Antibody detects endogenous levels of total RNase H1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RNase H1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Ribonuclease H1

Target: Ribonuclease H1 (RNASEH1)

Alternative Name: RNASEH1

Background:

Description: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (PubMed:10497183). Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II (PubMed:21700224).

Gene: RNASEH1

Molecular Weight: 32 kDa

Gene ID: 246243

UniProt: O60930