Ribonuclease H1 antibody (Middle Region)

Catalog No. ABIN2778825

This Rabbit Polyclonal antibody specifically detects Ribonuclease H1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Pig, Saccharomyces cerevisiae and Zebrafish (Danio rerio).

Quick Overview for Ribonuclease H1 antibody (Middle Region) (ABIN2778825)

Target: Ribonuclease H1 (RNASEH1)

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ribonuclease H1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Ribonuclease H1 Antibody

Binding Specificity: Middle Region

Purpose: RNASEH1 Antibody - middle region

Sequence: EVINKEDFVA LERLTQGMDI QWMHVPGHSG FIGNEEADRL AREGAKQSED

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against RNASEH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNASEH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40434-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Ribonuclease H1

Target: Ribonuclease H1 (RNASEH1)

Alternative Name: RNASEH1

Background:

Background Information: RNASEH1 belongs to the RNase H family. It contains 1 RNase H domain. RNASEH1 is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids.

Gene Name: Ribonuclease H1

Alternative Symbols: RNH1, H1RNA, PEOB2

Protein Name: Ribonuclease H1

Molecular Weight: 32 kDa

Gene ID: 246243

NCBI Accession: NP_002927

UniProt: O60930