RTCD1 antibody (C-Term)

Catalog No. ABIN6264878

This Rabbit Polyclonal antibody specifically detects RTCD1 in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for RTCD1 antibody (C-Term) (ABIN6264878)

Target: RTCD1 (RNA terminal Phosphate Cyclase Domain 1 (RTCD1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RTCD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RTCD1 Antibody

Binding Specificity: C-Term

Specificity: RTCD1 Antibody detects endogenous levels of total RTCD1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RTCD1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RTCD1

Target: RTCD1 (RNA terminal Phosphate Cyclase Domain 1 (RTCD1))

Alternative Name: RTCA

Background:

Description: Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP, (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A, (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.

Gene: RTCA

Molecular Weight: 34kDa

Gene ID: 8634

UniProt: O00442