SIAH2 antibody (C-Term)

Catalog No. ABIN6265080

The Rabbit Polyclonal anti-SIAH2 antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect SIAH2 in samples from Human, Mouse and Rat.

Quick Overview for SIAH2 antibody (C-Term) (ABIN6265080)

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SIAH2 Antibody

Binding Specificity: C-Term

Specificity: SIAH2 Antibody detects endogenous levels of total SIAH2.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SIAH2, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SIAH2

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Alternative Name: SIAH2

Background:

Description: Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.

Gene: MEN1

Molecular Weight: 36 kDa

Gene ID: 6478

UniProt: O43255