TRADD antibody (N-Term)

Catalog No. ABIN6265701

The Rabbit Polyclonal anti-TRADD antibody is suitable to detect TRADD in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF and ICC.

Quick Overview for TRADD antibody (N-Term) (ABIN6265701)

Target: TRADD (TNFRSF1A-Associated Via Death Domain (TRADD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRADD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-TRADD Antibody

Binding Specificity: N-Term

Specificity: TRADD Antibody detects endogenous levels of total TRADD.

Predicted Reactivity: Pig,Bovine,Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TRADD, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TRADD

Target: TRADD (TNFRSF1A-Associated Via Death Domain (TRADD))

Alternative Name: TRADD

Background:

Description: The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.

Gene: TRADD

Molecular Weight: 34kDa

Gene ID: 8717

UniProt: Q15628

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Hepatitis C