MID1 antibody (N-Term)

Catalog No. ABIN6265724

This Rabbit Polyclonal antibody specifically detects MID1 in WB, IHC, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MID1 antibody (N-Term) (ABIN6265724)

Target: MID1 (Midline 1 (MID1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MID1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MID1 Antibody

Binding Specificity: N-Term

Specificity: TRI18 Antibody detects endogenous levels of total TRI18.

Predicted Reactivity: Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TRI18, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC: 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MID1

Target: MID1 (Midline 1 (MID1))

Alternative Name: MID1

Background:

Description: Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.

Gene: MID1

Molecular Weight: 75kDa

Gene ID: 4281

UniProt: O15344