NPRL2 antibody (C-Term)

Catalog No. ABIN6265808

This Rabbit Polyclonal antibody specifically detects NPRL2 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NPRL2 antibody (C-Term) (ABIN6265808)

Target: NPRL2 (Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPRL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NPRL2 Antibody

Binding Specificity: C-Term

Specificity: TUSC4 Antibody detects endogenous levels of total TUSC4.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TUSC4, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NPRL2

Target: NPRL2 (Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2))

Alternative Name: NPRL2

Background:

Description: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.

Gene: NPRL2

Molecular Weight: 44 kDa

Gene ID: 10641

UniProt: Q8WTW4