UFM1 antibody (Internal Region)

Catalog No. ABIN6265872

The Rabbit Polyclonal anti-UFM1 antibody is suitable to detect UFM1 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for UFM1 antibody (Internal Region) (ABIN6265872)

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UFM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UFM1 Antibody

Binding Specificity: Internal Region

Specificity: UFM1 Antibody detects endogenous levels of total UFM1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human UFM1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for UFM1

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Alternative Name: UFM1

Background:

Description: Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to substrate proteins as a monomer or a lysine-linked polymer (PubMed:15071506, PubMed:20018847). The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1 (PubMed:15071506, PubMed:20018847). This post-translational modification on lysine residues of proteins may play a crucial role in a number of cellular processes (PubMed:15071506, PubMed:20018847). TRIP4 ufmylation may for instance play a role in nuclear receptors-mediated transcription (PubMed:25219498). Other substrates may include DDRGK1 with which it may play a role in the cellular response to endoplasmic reticulum stress (Probable).

Gene: UFM1

Molecular Weight: 9 kDa

Gene ID: 51569

UniProt: P61960