Mesothelin antibody (AA 37-296)

Catalog No. ABIN6290624

The Rabbit Polyclonal anti-Mesothelin antibody has been validated for WB, IHC and IF. It is suitable to detect Mesothelin in samples from Human and Rat.

Quick Overview for Mesothelin antibody (AA 37-296) (ABIN6290624)

Target: Mesothelin (MSLN)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Mesothelin Antibody

Binding Specificity: AA 37-296

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-296 of human MSLN (NP_001170826.1)

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Comment:

Expressed in lung, Expressed at low levels in heart, placenta and kidney, Expressed in mesothelial cells, Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level)

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20C. Avoid freeze / thaw cycles.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: MSLN

Background: This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Molecular Weight: 68.986 kDa

Gene ID: 10232

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process