WT1 antibody (AA 1-181)

Catalog No. ABIN6294230

The Mouse Monoclonal anti-WT1 antibody has been validated for IHC, IF and FACS. It is suitable to detect WT1 in samples from Human.

Quick Overview for WT1 antibody (AA 1-181) (ABIN6294230)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-WT1 Antibody

Binding Specificity: AA 1-181

Purpose: Mouse anti-Human Wilms Tumor 1 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic, membranous

Purification: A recombinant protein corresponding to residues 1-181 from the human protein was used as the immunogen for the Wilms Tumor 1 antibody.

Immunogen: A recombinant protein corresponding to residues 1-181 from the human protein was used as the immunogen for the Wilms Tumor 1 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wilms tumor protein

Background:

Target Description: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Gene Symbol: WT1

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation