SOX2 antibody (AA 176-305)

Catalog No. ABIN6294339

This anti-SOX2 antibody is a Mouse Monoclonal antibody detecting SOX2 in WB, ELISA, IHC, IF, FACS and Coat. Suitable for Human.

Quick Overview for SOX2 antibody (AA 176-305) (ABIN6294339)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-SOX2 Antibody

Binding Specificity: AA 176-305

Purpose: Mouse anti-Human SOX2 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic, nuclear

Purification: Amino acids 176-305 of the human protein were used as the immunogen for the SOX-2 antibody.

Immunogen: Amino acids 176-305 of the human protein were used as the immunogen for the SOX-2 antibody.

Application Details

Application Notes: ELISA: 2-4 μg/mL, order BSA-free formulation for coating
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: Transcription factor SOX-2

Background:

Target Description: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression.

Gene Symbol: SOX2

Gene ID: 6657

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis