MYOD1 antibody (AA 180-189)

Catalog No. ABIN6294345

This anti-MYOD1 antibody is a Mouse Monoclonal antibody detecting MYOD1 in IF, IHC and FACS. Suitable for Human, Mouse, Rat and Chicken.

Quick Overview for MYOD1 antibody (AA 180-189) (ABIN6294345)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-MYOD1 Antibody

Binding Specificity: AA 180-189

Purpose: Mouse anti-Human/Mouse/Rat/Chicken MyoD1 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Recombinant mouse protein was used as the immunogen for this anti-MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189.

Immunogen: Recombinant mouse protein was used as the immunogen for this anti-MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: Myoblast determination protein 1

Background:

Target Description: Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.

Gene Symbol: MYOD1///BHLHC1///MYF3///MYOD

Gene ID: 4654

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development