BCL6 antibody (AA 256-389)

Catalog No. ABIN6294426

This anti-BCL6 antibody is a Mouse Monoclonal antibody detecting BCL6 in IHC, FACS and IF. Suitable for Human.

Quick Overview for BCL6 antibody (AA 256-389) (ABIN6294426)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-BCL6 Antibody

Binding Specificity: AA 256-389

Purpose: Mouse anti-Human Bcl6 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Amino acids 256-389 from the human protein were used as the immunogen for the Bcl6 antibody.

Immunogen: Amino acids 256-389 from the human protein were used as the immunogen for the Bcl6 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: B-cell lymphoma 6 protein

Background:

Target Description: Antibody to Bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show Bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, Bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Gene Symbol: BCL6///BCL5///LAZ3///ZBTB27///ZNF51

Gene ID: 604

UniProt: P41182

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus