TFF1 antibody (AA 57-84)

Catalog No. ABIN6294557

The Mouse Monoclonal anti-TFF1 antibody has been validated for IHC, IF and FACS. It is suitable to detect TFF1 in samples from Human and Cynomolgus.

Quick Overview for TFF1 antibody (AA 57-84) (ABIN6294557)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reactivity: Human, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TFF1 Antibody

Binding Specificity: AA 57-84

Purpose: Mouse anti-Human/Cynomolgus Monkey pS2 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Amino acids 57-84 (CFDDTVRGVPWCFYPNTIDVPPEEECEF) from the human protein were used as the immunogen for this pS2 antibody.

Immunogen: Amino acids 57-84 (CFDDTVRGVPWCFYPNTIDVPPEEECEF) from the human protein were used as the immunogen for this pS2 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: Trefoil factor 1

Background:

Target Description: It recognizes a polypeptide of 6.5 kDa, identified as pS2 estrogen-regulated protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60 % of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

Gene Symbol: TFF1///BCEI///PS2

Gene ID: 7031

UniProt: P04155

Pathways: EGFR Signaling Pathway