TFF1 antibody (C-Term)

Catalog No. ABIN6294649

This Mouse Monoclonal antibody specifically detects TFF1 in IHC, IF and FACS. It exhibits reactivity toward Human and Cynomolgus.

Quick Overview for TFF1 antibody (C-Term) (ABIN6294649)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reactivity: Human, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TFF1 Antibody

Binding Specificity: C-Term

Purpose: Mouse anti-Human/Cynomolgus Monkey Estrogen Inducible Protein pS2 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen inducible protein pS2 was used as the immunogen for this antibody.

Immunogen: Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen inducible protein pS2 was used as the immunogen for this antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: Trefoil factor 1

Background:

Target Description: This antibody recognizes a polypeptide of ~6.5 kDa, identified as Estrogen Inducible Protein pS2. The antibody's epitope is localized between amino acids 57-84. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. Estrogen Inducible Protein pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60 % of breast carcinomas are positive for estrogen inducible protein pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that estrogen inducible protein pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

Gene Symbol: TFF1///BCEI///PS2

Gene ID: 7031

UniProt: P04155

Pathways: EGFR Signaling Pathway