N-Cadherin antibody (Cytoplasmic Domain)

Catalog No. ABIN6295444

This Mouse Monoclonal antibody specifically detects N-Cadherin in WB, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for N-Cadherin antibody (Cytoplasmic Domain) (ABIN6295444)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-N-Cadherin Antibody

Binding Specificity: Cytoplasmic Domain

Purpose: Mouse anti-Human/Mouse/Rat N-Cadherin Antibody / CDH2 [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A human partial recombinant protein corresponding to the CDH2/NCAD cytoplasmic domain was used as the immunogen for this N-Cadherin antibody.

Immunogen: A human partial recombinant protein corresponding to the CDH2/NCAD cytoplasmic domain was used as the immunogen for this N-Cadherin antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10^6 cells in 0.1ml
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: Cadherin-2

Background:

Target Description: Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CDH2 and CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. NCAD/CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, the protein has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, it is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Gene Symbol: CDH2///CDHN///NCAD

Gene ID: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane