PODXL antibody (Extracellular Domain)

Catalog No. ABIN6295450

This Mouse Monoclonal antibody specifically detects PODXL in WB, IHC, IP, FACS and IF. It exhibits reactivity toward Human, Rat and Rabbit.

Quick Overview for PODXL antibody (Extracellular Domain) (ABIN6295450)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This PODXL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-PODXL Antibody

Binding Specificity: Extracellular Domain

Purpose: Mouse anti-Human/Rabbit/Rat Podocalyxin Antibody [Sodium Azide Free]

Specificity: Cell surface and cytoplasmic

Purification: A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.

Immunogen: A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IP: 1-2 μg/500 μg protein
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: Podocalyxin

Background:

Target Description: Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Gene Symbol: PODXL///PCLP///PCLP1

Gene ID: 5420

UniProt: O00592

Pathways: Tube Formation