CLTA antibody (N-Term)

Catalog No. ABIN6295474

This Mouse Monoclonal antibody specifically detects CLTA in WB, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CLTA antibody (N-Term) (ABIN6295474)

Target: CLTA (Clathrin, Light Chain A (CLTA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLTA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-CLTA Antibody

Binding Specificity: N-Term

Purpose: Mouse anti-Human Clatherin Light Chain Antibody (LCA/LCB) [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody.

Immunogen: A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CLTA

Target: CLTA (Clathrin, Light Chain A (CLTA))

Alternative Name: Clathrin light chain A

Background:

Target Description: Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Gene Symbol: CLTA///CLTB

UniProt: P09496, P09497

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation