MITF antibody (N-Term)

Catalog No. ABIN6295495

This Mouse Monoclonal antibody specifically detects MITF in IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for MITF antibody (N-Term) (ABIN6295495)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-MITF Antibody

Binding Specificity: N-Term

Purpose: Mouse anti-Human MITF Antibody Cocktail [Sodium Azide Free]

Specificity: Nuclear

Purification: An N-terminus fragment (D5) and recombinant human protein (MITF/915) were used as the immunogen for the MITF antibody cocktail.

Immunogen: An N-terminus fragment (D5) and recombinant human protein (MITF/915) were used as the immunogen for the MITF antibody cocktail.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: Microphthalmia-associated transcription factor

Background:

Target Description: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

Gene Symbol: MITF///BHLHE32

Gene ID: 4286

UniProt: O75030

Pathways: Chromatin Binding