Cytokeratin 5 antibody

Catalog No. ABIN6296028

This anti-Cytokeratin 5 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 5 in WB, IHC, IF and FACS. Suitable for Human, Mouse, Rat, Cow, Pig, Monkey, Guinea Pig, Goat and Frog.

Quick Overview for Cytokeratin 5 antibody (ABIN6296028)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human, Mouse, Rat, Cow, Pig, Monkey, Guinea Pig, Goat, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Cytokeratin 5 Antibody

Purpose: Mouse anti-Human/Monkey/Cow/Pig/Goat/Rat/Mouse/Guinea Pig/Frog Multi Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the Multi Cytokeratin antibody.

Immunogen: A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the Multi Cytokeratin antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Keratin, type II cytoskeletal 5

Background:

Target Description: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Gene Symbol: KRT5///KRT6A///K6A///KRT6D///KRT8///CYK8///KRT10///KPP///KRT13///KRT18///CYK18///PIG46

UniProt: P13647, P02538, P05787, P13645, P13646, P05783