Nucleoli Marker antibody

Catalog No. ABIN6296065

This Mouse Monoclonal antibody specifically detects Nucleoli Marker in IF, FACS, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for Nucleoli Marker antibody (ABIN6296065)

Target: Nucleoli Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details

Purpose: Mouse anti-Human Nucleoli Marker Antibody [Sodium Azide Free]

Specificity: Nucleoli

Purification: A preparation of myeloid leukemia cell nuclei was used as the immunogen.

Immunogen: A preparation of myeloid leukemia cell nuclei was used as the immunogen.

Application Details

Application Notes: FACS: 0.5-1 μg/10^6 cells
IF: 0.5-1.0 μg/mL
Immunocytochemistry (Acetone-fixed): 0.5-1.0 μg/mL for 30 min at RT
IHC (Formalin-paraffin): 0.5-1.0 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details

Target: Nucleoli Marker

Background:

Target Description: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]