CTNNB1 antibody

Catalog No. ABIN6296117

The Rabbit Polyclonal anti-CTNNB1 antibody has been validated for WB, IHC, IF and FACS. It is suitable to detect CTNNB1 in samples from Human and Mouse.

Quick Overview for CTNNB1 antibody (ABIN6296117)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTNNB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-CTNNB1 Antibody

Purpose: Rabbit anti-Human/Mouse beta-Catenin Antibody [Sodium Azide Free]

Specificity: Cell surface and cytoplasmic

Purification: A synthetic peptide from the middle of beta-Catenin / p120 protein was used as the immunogen.

Immunogen: A synthetic peptide from the middle of beta-Catenin / p120 protein was used as the immunogen.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Alternative Name: Catenin beta-1

Background:

Target Description: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, it is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution throughout the cytoplasm results in a diffuse cytoplasmic pattern. Staining with beta-catenin antibody and E-cadherin antibody helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Gene Symbol: CTNNB1///CTNNB///OK///SW-cl.35///PRO2286

Gene ID: 1499

UniProt: P35222

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2