alpha Fetoprotein antibody

Catalog No. ABIN6296143

This Mouse Monoclonal antibody specifically detects alpha Fetoprotein in IHC, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for alpha Fetoprotein antibody (ABIN6296143)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-alpha Fetoprotein Antibody

Purpose: Mouse anti-Human/Mouse Alpha Fetoprotein Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: AFP purified from serum of a hepatoma patient was used as the immunogen for the Alpha Fetoprotein antibody.

Immunogen: AFP purified from serum of a hepatoma patient was used as the immunogen for the Alpha Fetoprotein antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: Alpha-fetoprotein

Background:

Target Description: It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Gene Symbol: AFP///HPAFP

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process