Keratin 77 antibody

Catalog No. ABIN6296176

This Rabbit Polyclonal antibody specifically detects Keratin 77 in IHC, WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Keratin 77 antibody (ABIN6296176)

Target: Keratin 77 (KRT77)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Keratin 77 Antibody

Purpose: Rabbit anti-Human Recombinant Acidic Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: An amino acid sequence common to acidic/type I keratins (1) was used as the immunogen for the recombinant Acidic Cytokeratin antibody.

Immunogen: An amino acid sequence common to acidic/type I keratins (1) was used as the immunogen for the recombinant Acidic Cytokeratin antibody.

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (3)
Prediluted IHC only format: incubate for 30 min at RT (2)
FACS: 0.5-1 μg/million cells in 0.1ml
WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: Keratin, type II cytoskeletal 1b

Background:

Target Description: There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]

Gene Symbol: KRT77///KRT1B

Gene ID: 374454

UniProt: Q7Z794