NEFL antibody

Catalog No. ABIN6296239

This Mouse Monoclonal antibody specifically detects NEFL in IHC, IF and FACS. It exhibits reactivity toward Human, Rat, Pig, Cow and Chicken.

Quick Overview for NEFL antibody (ABIN6296239)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human, Rat, Pig, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-NEFL Antibody

Purpose: Mouse anti-Human/Rat/Pig/Cow/Chicken Neurofilament Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the Neurofilament antibody.

Immunogen: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the Neurofilament antibody.

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament light polypeptide

Background:

Target Description: This mAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Gene Symbol: NEFL///NF68///NFL

Gene ID: 4747

UniProt: P07196