NEFH antibody

Catalog No. ABIN6296241

This anti-NEFH antibody is a Mouse Monoclonal antibody detecting NEFH in IHC and FACS. Suitable for Human, Rat, Mouse, Chicken, Pig, Cow, Rabbit, Guinea Pig, Gerbil and Cat.

Quick Overview for NEFH antibody (ABIN6296241)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Mouse, Chicken, Pig, Cow, Rabbit, Guinea Pig, Gerbil, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-NEFH Antibody

Purpose: Mouse anti-Human/Mouse/Rat/Guinea pig/Gerbil/Cat/Pig/Rabbit/Cow/Chicken NF-H Antibody (phospho) [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).

Immunogen: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: Neurofilament heavy polypeptide

Background:

Target Description: This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Gene Symbol: NEFH///KIAA0845///NFH

Gene ID: 4744

UniProt: P12036