NKX2-1 antibody

Catalog No. ABIN6296286

This anti-NKX2-1 antibody is a Mouse Monoclonal antibody detecting NKX2-1 in IHC, FACS and IF. Suitable for Human, Mouse and Rat.

Quick Overview for NKX2-1 antibody (ABIN6296286)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NKX2-1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-NKX2-1 Antibody

Purpose: Mouse anti-Human/Mouse/Rat NKX2.1 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Full length rat recombinant protein was used as the immunogen for this NKX2.1 antibody.

Immunogen: Full length rat recombinant protein was used as the immunogen for this NKX2.1 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Alternative Name: Homeobox protein Nkx-2.1

Background:

Target Description: Recognizes a protein of 40 kDa, identified as NKX2.1 or Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. Reactivity is also seen in thyroid malignancies.

Gene Symbol: NKX2-1///NKX2A///TITF1///TTF1

Gene ID: 7080

UniProt: P43699

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour