Cyclin B1 antibody

Catalog No. ABIN6296320

This Mouse Monoclonal antibody specifically detects Cyclin B1 in IF, FACS, IP and BP. It exhibits reactivity toward Human, Mouse and Hamster.

Quick Overview for Cyclin B1 antibody (ABIN6296320)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)

Product Details anti-Cyclin B1 Antibody

Purpose: Mouse anti-Human/Mouse/Hamster Cyclin B1 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Hamster protein was used as the immunogen for this Cyclin B1 antibody.

Immunogen: Hamster protein was used as the immunogen for this Cyclin B1 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IP: 1-2 μg/500 μg protein--precipitates active Cyclin B1 complexes

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: G2/mitotic cyclin-B1

Background:

Target Description: This antibody recognizes a protein of 55-62 kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.

Gene Symbol: CCNB1///CCNB

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase