Cytokeratin 1 antibody (PE)

Catalog No. ABIN6296411

The Mouse Monoclonal anti-Cytokeratin 1 antibody has been validated for FACS, IF and T. It is suitable to detect Cytokeratin 1 in samples from Human, Rat, Mouse, Cow, Rabbit, Chicken, Monkey and Dog.

Quick Overview for Cytokeratin 1 antibody (PE) (ABIN6296411)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Chicken, Monkey, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Product Details anti-Cytokeratin 1 Antibody (PE)

Purpose: Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken Pan Cytokeratin Antibody Cocktail [PE Conjugated]

Specificity: Cytoplasmic

Purification: Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody.

Immunogen: Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody.

Application Details

Application Notes: Flow Cytometry : 5 µL/test/million cells or 5 µL/test/100 µL of whole blood
Immunofluorescence: 1:50-1:100 for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4) with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C. The conjugate is light-sensitive, protect from light.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Keratin, type II cytoskeletal 1

Background:

Target Description: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis. The AE1 + AE3 antibody cocktail is a broad spectrum pan cytokeratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. It stains cytokeratin present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Gene Symbol: KRT1///KRTA///KRT3///KRT3///KRT5///KRT6A///K6A///KRT6D///KRT8///CYK8///KRT10///KPP///KRT14///KRT15///KRTB///KRT16///KRT16A

UniProt: P04264, P12035, P13647, P02538, P05787, P13645, P02533, P19012, P08779, P08727