CD35 antibody

Catalog No. ABIN6296464

This Mouse Monoclonal antibody specifically detects CD35 in FACS, IHC and IF. It exhibits reactivity toward Human, Baboon, Cynomolgus and Rhesus Monkey.

Quick Overview for CD35 antibody (ABIN6296464)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human, Baboon, Cynomolgus, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD35 Antibody

Purpose: Mouse anti-Human/Baboon/Cynomolgus Monkey/Rhesus Monkey CD35 Antibody (Complement Receptor 1) [Sodium Azide Free]

Specificity: Cell surface

Purification: Human monocytes were used as the immunogen for this CD35 antibody.

Immunogen: Human monocytes were used as the immunogen for this CD35 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: Complement receptor type 1

Background:

Target Description: Recognizes a protein of 210-220 kDa, which is identified as the Complement receptor 1 or CD35. This mAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1/CD35 activity. It is highly specific and shows no cross-reaction with CR2/CD21. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This mAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

Gene Symbol: CR1///C3BR

Gene ID: 1378

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity