S100A9 antibody

Catalog No. ABIN6296494

This Mouse Monoclonal antibody specifically detects S100A9 in WB, IHC, IF and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for S100A9 antibody (ABIN6296494)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-S100A9 Antibody

Purpose: Mouse anti-Human/Rat S100A9 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic, extracellular (secreted) and cell surface

Purification: Human peripheral blood monocyte components were used as the immunogen for the S100A9 antibody.

Immunogen: Human peripheral blood monocyte components were used as the immunogen for the S100A9 antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: Protein S100-A9

Background:

Target Description: This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Gene Symbol: S100A9///CAGB///CFAG///MRP14

Gene ID: 6280

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins