Macrophages antibody

Catalog No. ABIN6296503

This Mouse Monoclonal antibody specifically detects Macrophages in FACS, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Macrophages antibody (ABIN6296503)

Target: Macrophages

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Purpose: Mouse anti-Human Macrophage Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Human peripheral blood mononuclear cells were used as the immunogen for the Macrophage antibody.

Immunogen: Human peripheral blood mononuclear cells were used as the immunogen for the Macrophage antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.): 1-2 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details

Target: Macrophages

Alternative Name: Macrophage

Background:

Target Description: This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer s cells of the liver and Langerhan s cells of the skin. Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and pro-monocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). LN-5 selectively stains human sebaceous glands in formalin-fixed, paraffin-embedded skin samples. Undifferentiated sebocyte progenitors are negative, and only sebocytes from the onset of their differentiation reveal positive cytoplasmic staining. Since there are very few selective and easy-to-use markers of sebaceous glands, LN-5 antibody can offer a simple and relatively specific way to detect human sebocytes from the onset of their.