Thymidine Phosphorylase antibody

Catalog No. ABIN6296537

The Mouse Monoclonal anti-Thymidine Phosphorylase antibody has been validated for WB, IHC and IP. It is suitable to detect Thymidine Phosphorylase in samples from Human, Rat and Mouse.

Quick Overview for Thymidine Phosphorylase antibody (ABIN6296537)

Target: Thymidine Phosphorylase (TYMP)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thymidine Phosphorylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-Thymidine Phosphorylase Antibody

Purpose: Mouse anti-Human/Mouse/Rat Thymidine Phosphorylase Antibody / PD-ECGF [Sodium Azide Free]

Specificity: Cytoplasmic, nuclear

Purification: Human recombinant full length protein was used as the immunogen for this Thymidine Phosphorylase antibody.

Immunogen: Human recombinant full length protein was used as the immunogen for this Thymidine Phosphorylase antibody.

Application Details

Application Notes: IP: 1-2 μg/500 μg lysate
IHC (FFPE): 1-2 μg/mL for 30 min at RT
WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Alternative Name: Thymidine phosphorylase

Background:

Target Description: Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Gene Symbol: TYMP///ECGF1

Gene ID: 1890

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2