Granulocytes antibody
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- Target
- Granulocytes
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Reactivity
- Human, Macaque
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- Mouse anti-Human/Macaque Monkey Granulocyte Antibody [Sodium Azide Free]
- Specificity
- Cytoplasmic
- Purification
- Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this Granulocyte antibody.
- Immunogen
- Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this Granulocyte antibody.
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anti-Granulocytes antibody
Reactivity: Human, Macaque FACS, IHC (p), IF Host: Mouse Monoclonal SPM250 unconjugated
anti-Granulocytes antibody (Biotin)Reactivity: Rat FACS, IHC (p), IHC (fro) Host: Mouse Monoclonal RK-4 Biotin
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- Application Notes
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Buffer
- In 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Target
- Granulocytes
- Alternative Name
- Granulocyte
- Background
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Target Description: Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. SPM250 Ab reacts with early precursor and mature forms of human myeloid cells. This mAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
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