Histone H1 antibody

Catalog No. ABIN6296764

This Mouse Monoclonal antibody specifically detects Histone H1 in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Histone H1 antibody (ABIN6296764)

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Histone H1 Antibody

Purpose: Mouse anti-Human/Mouse/Rat Histone Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.

Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.

Application Details

Application Notes: Western blot: 0.5-1 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Histone H1

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Alternative Name: Histone H1.0

Background:

Target Description: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Gene Symbol: H1F0///H1FV

Gene ID: 3005

UniProt: P07305