Nuclear Antigen antibody

Catalog No. ABIN6296766

The Mouse Monoclonal anti-Nuclear Antigen antibody (ABIN6296766) specifically detects Nuclear Antigen in IF, FACS, ICC and IHC (fro). The antibody is reactive with Human and Primate samples.

Quick Overview for Nuclear Antigen antibody (ABIN6296766)

Target: Nuclear Antigen

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details

Purpose: Mouse anti-Human/Primates Nuclear Antigen Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear antigen antibody.

Immunogen: Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear antigen antibody.

Application Details

Application Notes: IHC (Frozen): 0.5-1 μg/mL for 30 minutes at RT (1)
Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT
FACS: 0.5-1 μg/10e6 cells
IF: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details

Target: Nuclear Antigen

Background:

Target Description: This nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.