Transgelin antibody

Catalog No. ABIN6297094

This Mouse Monoclonal antibody specifically detects Transgelin in IHC, FACS and IF. It exhibits reactivity toward Human, Mouse, Rabbit, Cow and Pig.

Quick Overview for Transgelin antibody (ABIN6297094)

Target: Transgelin (TAGLN)

Reactivity: Human, Mouse, Rabbit, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transgelin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Transgelin Antibody

Purpose: Mouse anti-Human/Cow/Pig/Rabbit/Mouse SM22 alpha Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant full-length human protein was used as the immunogen for the SM22 antibody.

Immunogen: Recombinant full-length human protein was used as the immunogen for the SM22 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Transgelin

Target: Transgelin (TAGLN)

Alternative Name: Transgelin

Background:

Target Description: This mAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

Gene Symbol: TAGLN///SM22///WS3-10

Gene ID: 6876

UniProt: Q01995