Transglutaminase 2 antibody

Catalog No. ABIN6297095

The Mouse Monoclonal anti-Transglutaminase 2 antibody is suitable to detect Transglutaminase 2 in samples from Human, Mouse, Rat, Monkey and Rabbit. It has been validated for IHC, IF and FACS.

Quick Overview for Transglutaminase 2 antibody (ABIN6297095)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Transglutaminase 2 Antibody

Purpose: Mouse anti-Human/Monkey/Rabbit/Mouse/Rat TGM2 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and cell surface

Purification: Recombinant full-length human protein was used as the immunogen for the TGM2 antibody.

Immunogen: Recombinant full-length human protein was used as the immunogen for the TGM2 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: Protein-glutamine gamma-glutamyltransferase 2

Background:

Target Description: Recognizes a 77-85 kDa protein, identified as cellular or tissue transglutaminase II (TGase II, TGM2). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Gene Symbol: TGM2

Gene ID: 7052

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling