ALPL antibody

Catalog No. ABIN6297134

The Mouse Monoclonal anti-ALPL antibody has been validated for IHC, FACS and IF. It is suitable to detect ALPL in samples from Human and Cow.

Quick Overview for ALPL antibody (ABIN6297134)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-ALPL Antibody

Purpose: Mouse anti-Human/Cow Alkaline Phosphatase Antibody (tissue-nonspecific) [Sodium Azide Free]

Specificity: Cell surface, cytoplasmic, secreted

Purification: Recombinant human ALPL protein was used as the immunogen for the Alkaline Phosphatase antibody.

Immunogen: Recombinant human ALPL protein was used as the immunogen for the Alkaline Phosphatase antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT
Prediluted format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: Alkaline phosphatase, tissue-nonspecific isozyme

Background:

Target Description: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Gene Symbol: ALPL

Gene ID: 249

UniProt: P05186