Desmin antibody

Catalog No. ABIN6297155

The Mouse Monoclonal anti-Desmin antibody has been validated for WB, IHC, IF and FACS. It is suitable to detect Desmin in samples from Human, Rat, Mouse, Chicken and Hamster.

Quick Overview for Desmin antibody (ABIN6297155)

Target: Desmin (DES)

Reactivity: Human, Rat, Mouse, Chicken, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Desmin Antibody

Purpose: Mouse anti-Human/Mouse/Rat/Hamster/Chicken Desmin Antibody (Muscle cell marker) [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant human Desmin was used as the immunogen for the Desmin antibody.

Immunogen: Recombinant human Desmin was used as the immunogen for the Desmin antibody.

Application Details

Application Notes: WB: 0.5-1 μg/mL
FACS: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin

Background:

Target Description: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Gene Symbol: DES

Gene ID: 1674

UniProt: P17661