Cyclin B1 antibody

Catalog No. ABIN6297159

This anti-Cyclin B1 antibody is a Mouse Monoclonal antibody detecting Cyclin B1 in IHC, IF and FACS. Suitable for Human and Mouse.

Quick Overview for Cyclin B1 antibody (ABIN6297159)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Cyclin B1 Antibody

Purpose: Mouse anti-Human/Mouse CCNB1 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic & nuclear

Purification: Recombinant human full-length protein was used as the immunogen for the CCNB1 antibody.

Immunogen: Recombinant human full-length protein was used as the immunogen for the CCNB1 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: G2/mitotic cyclin-B1

Background:

Target Description: It recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Gene Symbol: CCNB1///CCNB

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase