Keratin 10 antibody

Catalog No. ABIN6297212

The Mouse Monoclonal anti-Keratin 10 antibody has been validated for IHC, FACS and IF. It is suitable to detect Keratin 10 in samples from Human and Mouse.

Quick Overview for Keratin 10 antibody (ABIN6297212)

Target: Keratin 10 (KRT10)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Keratin 10 Antibody

Purpose: Mouse anti-Human/Mouse Keratin 10 Antibody (KRT10) [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody.

Immunogen: Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Keratin, type I cytoskeletal 10

Background:

Target Description: This mAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Gene Symbol: KRT10///KPP

Gene ID: 3858

UniProt: P13645