MLANA antibody

Catalog No. ABIN6297244

This Mouse Monoclonal antibody specifically detects MLANA in WB, IF, FACS, IHC, ELISA, IP and Coat. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MLANA antibody (ABIN6297244)

Target: MLANA (Melan A (MLANA))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Coating (Coat)

Product Details anti-MLANA Antibody

Purpose: Mouse anti-Human/Mouse/Rat Melan-A Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant human Melan-A protein was used as the immunogen for this antibody.

Immunogen: Recombinant human Melan-A protein was used as the immunogen for this antibody.

Application Details

Application Notes: ELISA: order BSA-free format for coating
FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IP: 0.5-1 μg/500 μg protein lysate
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: Melanoma antigen recognized by T-cells 1

Background:

Target Description: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Gene Symbol: MLANA///MART1

Gene ID: 2315

UniProt: Q16655