MLANA antibody

Catalog No. ABIN6297246

The Mouse Monoclonal anti-MLANA antibody (ABIN6297246) specifically detects MLANA in WB, IF, FACS and IHC. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for MLANA antibody (ABIN6297246)

Target: MLANA (Melan A (MLANA))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-MLANA Antibody

Purpose: Mouse anti-Human/Mouse/Rat/Dog Melan-A Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant human MLANA protein was used as the immunogen for the Melan-A antibody.

Immunogen: Recombinant human MLANA protein was used as the immunogen for the Melan-A antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Western blot: 0.5-1 μg/mL
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: Melanoma antigen recognized by T-cells 1

Background:

Target Description: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Gene Symbol: MLANA///MART1

Gene ID: 2315

UniProt: Q16655